Human Whole Genome Sequencing

Human whole genome sequencing (hWGS) enables researchers to catalog a genetic constitution of individuals and capture all variants present in a single assay. It is utilized to study cancer and a variety of diseases, as well as human population evolution studies and pharmacogenomics.

Equipped with the powerful Illumina NovaSeq 6000 system, Novogene is capable of sequencing up to 280,000 human genomes per year at the lowest cost per genome. With extensive experience in whole genome sequencing and advanced bioinformatics capabilities, Novogene is able to expertly meet customer needs for delivering large project results with quick turnaround times and the highest quality results.

Novogene Advantage

Scalable Sequencing Capacity

Industry-Leading Data Quality Guarantee (Q30 > 85%)

Competitive Pricing

Extensive In-House Bioinformatics Expertise

Project Workflow

Sequencing Speciﬁcations

Sample Requirements

DNA Amount: ≥ 200 ng (gDNA from fresh tissues); ≥ 0.8 μg (gDNA from FFPE)
DNA Concentration: ≥ 10 ng/μL
Purity: OD 260/280 = 1.8-2.0; no degradation and no contamination (RNA or protein)

Sequencing Strategy

350 bp insert DNA library
NovaSeq 6000 platform, PE150

Recommended Sequencing Depth

For tumor tissues: 50×, adjacent normal tissues and blood 30×
For rare diseases: 30-50×

Turnaround Time

11 working days after verification of sample quality without data analysis (depending on sample size)

Analysis Pipeline

* Detailed items on advanced analysis to suit your project are available. Please contact us for more information.

Novogene Powered Publications

Human Whole Exome Sequencing

Exome sequencing provides a cost-effective alternative to whole genome sequencing, as it targets only the protein coding region of the human genome responsible for a majority of known disease-related variants. Whether you are conducting studies in rare mendelian disorders, complex disease, cancer research, or human population studies, Novogene’s comprehensive human whole exome sequencing (hWES) service provides a high-quality, affordable, and convenient solution.

Advantages of hWES

Targeted Protein Coding Region:

by capturing and sequencing protein coding region, hWES is utilized to reveal variants related to protein structure

High Accuracy:

with high sequencing depth, hWES facilitates detection of common variants and rare variants with frequencies lower than 1%

Cost Effective:

hWES yields approximately 85% of human disease mutations from 1% of human genome

Project Workflow

Sequencing Speciﬁcations

Exome Capture

Agilent SureSelect Human All Exon V6 Kit

Sequencing Strategy

NovaSeq 6000 platform, PE150

Sample Requirements

DNA Amount: ≥ 400 ng (gDNA from fresh tissues); ≥ 0.8 μg (gDNA from FFPE)
DNA Concentration: ≥ 20 ng/μL
Purity: D260/280 = 1.8-2.0; no degradation and no contamination (RNA or protein)

Recommended Sequencing Depth