Novogene - Shipping Instructions for 10x scRNA-seq

Using freshly collected samples is preferred. It is recommended to rinse freshly extracted tissue sample from living animals immediately, with precooled RNase-free water. Ensure removal of blood, unwanted tissues (such as hair and connective tissue) and other contaminants.

Sending in snap/fresh-frozen

Quickly cut tissues into smaller pieces (50~100 mg) on ice and freeze using liquid nitrogen. Store sample in pre-chilled RNase-free 1.5 mL/2.0 mL EP tubes or freezing tubes with a screw cap. Seal tubes with sealing film and stored at-80°C immediately. To ensure RNA preservation, it is recommended to complete this step within 3 minutes upon tissue removal from living body. Aliquot and store sample into several tubes to avoid degradation due to repeated freezing and thawing.

Sending in fresh(recommended)

If sending fresh organ or tissue samples without being snap-frozen, please use commercial RNA protection reagent (recommend MACS^® Tissue Storage Solution) and follow manufacturer recommendation. (Shipping with ice packs for fresh tissues)

For more information, please contact Novogene representatives.

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