August 1st marks the annual RNA Day, a celebration of one of the three vital biological molecules for all known forms of life, the ribonucleic acid (RNA). Empowered with the accurate next-generation sequencing (NGS) technologies, RNA sequencing (RNA-seq) is a powerful technique for investigating and discovering the continuous changes occurring in cellular transcriptome, and allows researchers to gain deeper understandings of cell biology and assess variations that may indicate disease.